Abstract Background and objectives: Nerve block or neurolysis is an important approach in the treatment of spastic equinovarus foot. To illustrate the accurate location of the nerve branch to the tibialis posterior muscle (TP) in clinical practice, 21 adult cadavers were dissected and 14 complete both lower limb specimens were obtained. A total of 28 lower limbs were included. Methods: We measured the length of the motor branch nerve (LM) of the tibialis posterior muscle, the length of the fibula (LF), the vertical distance (D1) from the midpoint of LM to the fibula tip as well as the horizontal distance (D2) from the midpoint of LM to the inner edge of the fibula. Results: The LM was higher (35.74 ± 7.28 mm) in male than in female (30.40 ± 6.88 mm) specimens but there was no significant correlation between LM and gender (> 0.05). Additionally, among male specimens, the LM on the right side was longer than that on the left (p ≤ 0.05) while among female specimens, the D1 on the left side was longer than that on the right (p ≤ 0.05). The LF in male specimen was significantly longer than that in female (p ≤ 0.05). The midpoint of the nerve to the motor branch of the tibialis posterior muscle was about 50 mm distal to the fibular head and 10 mm at the inner edge of the fibula. Conclusion: Using this coordinate, the midpoint of the nerve branch to the TP could be accurately located.

Abstract The purpose of this study was to observe its in vitro inhibitory effect on human thyroid cancer cell line SW579 through PI3K-Akt pathway. In this study, the survival of cells and the inhibitory effect of gardenia tea extract (GTE) on SW579 cancer cells were firstly observed by MTT method. Further qPCR experiments were used to observe mRNA expression in SW579 cancer cells. The active compound composition in the GTE was then also determined by HPLC. The experimental results showed that GTE had almost no effect on normal primary thyroid cells and had no toxic effect, but GTE could significantly inhibit the proliferation of SW579 cancer cells. The detection of mRNA expression in SW579 cancer cells showed that GTE could up-regulate the expressions of Bax, caspase-3, caspase-8 and caspase-9 and down-regulate the expressions of Bcl-2, Bcl-xL, PI3K and Akt in SW579 cancer cells. The chemical composition test showed that GTE mainly contains three compounds, namely rutin, quercetin and phloretin. It can be seen that gardenia tea can regulate the PI3K-Akt pathway of SW579 cancer cells through the effective active substances contained in it, and exert its anti-cancer effect. It is a health-care tea with good anti-cancer effect.